donkey anti mouse dylight 550 sa5-10167  (Thermo Fisher)

Journal: iScience

Article Title: Homozygous ALS-linked mutations in TARDBP/TDP-43 lead to hypoactivity and synaptic abnormalities in human iPSC-derived motor neurons

doi: 10.1016/j.isci.2024.109166

Figure Lengend Snippet:

Article Snippet: Donkey anti-Mouse IgG DyLight® 550 , Abcam , Cat#ab96876; RRID: AB_10679663.

Techniques: Recombinant, Gentle, Cell Viability Assay, DC Protein Assay, Western Blot, Sequencing, Software

Journal: Microbial Cell Factories

Article Title: A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins

doi: 10.1186/s12934-023-02200-4

Figure Lengend Snippet: Surface display and secretion of Fab-AH3/E3 in diploid Y-AH3/E3. a . The galactose induced yeast cells were harvested and stained to detect Fab display by FACS. The cells were incubated with mouse anti-HA and goat anti-human kappa followed by secondary labeling with donkey anti-mouse Dylight 550 and donkey anti-goat AF 488. The non-induced cells were used as a negative control. b. Neutralization assay with secretion culture supernatant. The yeast cells were induced to secrete Fab in SD medium containing 1% or 2% glucose at 30 °C for 72 h. The supernatants were diluted 4 times and co-cultured with Vero cells in the presence of 50 ng/mL TcdA or 10 pg/mL TcdB at 37 °C overnight. The images were taken using a phase-contrast microscope

Article Snippet: To evaluate surface display by flow cytometry, the cells were incubated with mouse anti-HA (Abcam, ab18181, 1:1000) and goat anti-human KC (Southern Biotech, 2060-01, 1:1000) followed by secondary labeling with donkey anti-mouse Dylight 550 (Invitrogen, SA5-10167, 1:500) and donkey anti-goat Alexa Fluor (AF) 488 (Invitrogen, A-11,055, 1:500).

Techniques: Staining, Incubation, Labeling, Negative Control, Neutralization, Cell Culture, Microscopy